| Corporate Office: 323-389-6028

Biostimulators & Biologics in Facial Rejuvenation : Providers Guide

  • Home
  • /
  • Education
  • /
  • Biostimulators & Biologics in Facial Rejuvenation : Providers Guide
  • 29

    In the quest for eternal youth and flawless skin, the aesthetics industry continually evolves, introducing innovative techniques and treatments.

    Amidst a myriad of treatments and techniques, two distinct categories have emerged as game-changers: biostimulators and biologics.

    MeDinjecT automated injector being used for facial rejuvenation treatment.
    Utilizing biostimulators or biologics in facial rejuvenation therapies offers innovative methods to enhance collagen production, promote skin renewal, and achieve a revitalized, youthful appearance.

    While these terms may sound similar, they represent distinct approaches to achieving the same goal – revitalizing the skin and restoring its natural radiance. The primary difference between a biostimulator and a biologic used in facial rejuvenation lies in their mechanisms of action and the substances they utilize to achieve skin rejuvenation.

    Let’s unravel the differences between biostimulators and biologics, exploring their unique mechanisms, benefits, and applications for facial rejuvenation.

    Biostimulators : Unveiling the Power of Collagen Induction

    Biostimulators are agents designed to stimulate the body’s natural processes, particularly collagen production. Collagen, the building block of youthful skin, provides structure, elasticity, and resilience. However, as we age, collagen production diminishes, leading to wrinkles, sagging, and loss of volume. Biostimulators intervene by jumpstarting collagen synthesis, effectively rejuvenating the skin from within.

    Mechanism: Biostimulators typically contain synthetic or biocompatible materials, such as poly-L-lactic acid (PLLA) or calcium hydroxylapatite (CaHA). When injected into the skin, these substances trigger a cascade of cellular responses, prompting fibroblasts to produce new collagen fibers. Over time, this process leads to improved skin texture, firmness, and overall youthfulness.

    Benefits: Biostimulators offer gradual yet long-lasting results, with improvements becoming more apparent over several weeks to months. Unlike traditional fillers, which provide immediate volume enhancement, biostimulators deliver a natural-looking outcome that evolves with time. Additionally, biostimulators can address a wide range of concerns, including wrinkles, folds, and facial contours, making them a versatile option for facial rejuvenation.

    Biostimulators Used for Facial Rejuvenation

    Calcium Hydroxylapatite (CaHA): Calcium hydroxylapatite, or CaHA, is a biostimulator commonly used for facial rejuvenation. CaHA is a mineral-like compound found naturally in bones, making it biocompatible and well-tolerated by the body.

    When injected into the skin, CaHA microspheres stimulate collagen production while providing immediate volume and lift. CaHA biostimulators are ideal for restoring facial contours, smoothing moderate to severe wrinkles, and enhancing overall facial harmony.

    The results are both immediate and long-lasting, with effects lasting up to 12 months or more.

    Poly-L-Lactic Acid (PLLA): Poly-L-lactic acid, commonly known as PLLA, is a biocompatible and biodegradable synthetic polymer.

    When injected into the skin, PLLA stimulates the production of collagen, gradually improving skin texture, volume, and elasticity. PLLA-based biostimulators are particularly effective for treating deep wrinkles, facial folds, and areas of volume loss.

    Unlike traditional fillers, which provide immediate results, PLLA biostimulators work gradually, with optimal outcomes typically visible after several months. The results are long-lasting, lasting up to two years or more in some cases.

    Close-up of automated injector being used to inject biostimulators into a patients face for facial rejuvenation.
    Biostimulators are increasingly administered using an automated injector to harness the body's natural processes to stimulate collagen production and restore youthful vitality to the skin.

    Polycaprolactone (PCL): Polycaprolactone, or PCL, is a biodegradable polyester that has gained popularity as a biostimulator for facial rejuvenation.

    PCL biostimulators stimulate collagen synthesis and provide structural support to the skin, resulting in improved firmness, elasticity, and texture. PCL injections are suitable for treating nasolabial folds, marionette lines, and other signs of aging.

    The results are gradual, with optimal improvement visible over several months. PCL biostimulators offer long-lasting effects, lasting up to 18 months or more.

    Polylactic-Co-Glycolic Acid (PLGA): Polylactic-co-glycolic acid, or PLGA, is a biodegradable copolymer that acts as a biostimulator for facial rejuvenation.

    PLGA microparticles are injected into the skin to stimulate collagen production and improve skin texture and elasticity. PLGA biostimulators are effective for treating fine lines, wrinkles, and areas of volume loss.

    The results of PLGA treatments are gradual and can last for several months.

    Biostimulator treatments offer a versatile and effective approach to facial rejuvenation, addressing a wide range of concerns, from wrinkles and fine lines to volume loss and skin laxity. By stimulating collagen production and promoting tissue regeneration, biostimulators help restore youthfulness and vitality to the skin, yielding natural-looking and long-lasting results.

    Whether you decide to administer PLLA, CaHA, PCL, PLGA or a combination of these biostimulators to your patients, these treatments can help them achieve radiant, refreshed, and rejuvenated skin, allowing them to look and feel their best at any age.

    Biologics : Tapping into the Body's Regenerative Potential

    Biologics represent a different approach to facial rejuvenation, focusing on harnessing the regenerative power of the body’s own tissues and proteins. Derived from natural sources such as blood, fat, or placental tissue, biologics contain bioactive substances that promote tissue repair, collagen synthesis, and overall skin renewal.

    Mechanism: Biologics, such as Platelet-Rich Plasma (PRP) or stem cell-based therapies, work in facial rejuvenation by stimulating the body’s natural healing processes. PRP contains growth factors and cytokines that promote collagen production, tissue repair, and angiogenesis, leading to improved skin texture, elasticity, and overall rejuvenation. Stem cell-based therapies utilize the regenerative potential of stem cells to repair damaged tissues, increase collagen synthesis, and enhance skin quality.

    Benefits: Biologics offer a natural and minimally invasive approach to facial rejuvenation, with little to no risk of allergic reactions or adverse side effects. These treatments can effectively address a range of concerns, including fine lines, wrinkles, scars, and uneven skin tone. Additionally, biologics promote tissue regeneration and healing, resulting in a fresher, more youthful complexion over time.

    Biologics Used for Facial Rejuvenation

    Exosomes: Exosomes are tiny membrane-bound vesicles secreted by cells that contain a variety of bioactive molecules, including growth factors, cytokines, and nucleic acids.

    For facial rejuvenation, exosomes derived from stem cells or other cellular sources are used to promote tissue regeneration, stimulate collagen production, and improve overall skin health.

    Exosome therapy offers a natural and minimally invasive approach to facial rejuvenation, with little to no risk of adverse reactions.

    Growth Factors: Growth factors are naturally occurring proteins that play a crucial role in regulating cell growth, proliferation, and differentiation.

    For facial rejuvenation, growth factors derived from sources such as human fibroblasts or placental tissue are used to promote tissue repair and stimulate collagen synthesis. By applying growth factors topically or incorporating them into cosmetic products, patients can achieve smoother, firmer, and more youthful-looking skin.

    Growth factor-based treatments are often used in conjunction with other facial rejuvenation procedures to enhance results and accelerate healing.

    Hyaluronic Acid: While not strictly a biologic, hyaluronic acid is a naturally occurring substance found in the skin that plays a crucial role in maintaining hydration and volume.

    For facial rejuvenation, hyaluronic acid fillers are commonly used to restore volume, fill in wrinkles and fine lines, and enhance facial contours.

    Hyaluronic acid fillers provide immediate results and are well-tolerated by most patients.

    Close-up of the biologic platelet rich plasma being injected into a patient's face using an automated injector.
    Biologics such as Platelet-Rich Plasma (PRP) are revolutionizing facial rejuvenation by harnessing the regenerative power of one's own blood components to stimulate tissue repair, enhance skin texture, and promote a youthful glow.

    Platelet-Rich Plasma (PRP): Platelet-rich plasma (PRP) is one of the most well-known and widely used biologics for facial rejuvenation. PRP is derived from the patient’s own blood, which is processed to concentrate platelets and growth factors.

    When injected into the skin, PRP stimulates collagen production, accelerates tissue repair, and improves overall skin texture and tone. PRP treatments are particularly effective for reducing fine lines and wrinkles, enhancing skin elasticity and restoring a youthful glow.

    The procedure is minimally invasive and involves little to no downtime, making it a popular choice for patients seeking natural-looking results with minimal risk.

    Stem Cell Therapy: Stem cell therapy is another promising biologic approach to facial rejuvenation. Stem cells are undifferentiated cells with the remarkable ability to differentiate into various cell types and promote tissue regeneration.

    For facial rejuvenation, stem cell therapy involves harvesting stem cells from the patient’s own adipose tissue (fat) or bone marrow, or harvesting them from placental tissues and introducing them into the skin through injections or topical application. Stem cell therapy promotes collagen production, improves skin elasticity, and enhances overall skin quality.

    While stem cell therapy is still a relatively novel treatment, ongoing research suggests promising outcomes in the field of aesthetic medicine.

    Biologics represent a paradigm shift in the field of facial rejuvenation, offering a natural and holistic approach to skin renewal and anti-aging. Whether through platelet-rich plasma, stem cell therapy, or growth factor-based treatments, biologics harness the body’s own regenerative mechanisms to restore youthfulness and vitality to the skin.

    As the demand for safe and effective cosmetic procedures continues to rise, biologics stand at the forefront of innovation, providing patients with cutting-edge solutions for achieving radiant, rejuvenated skin.

    Treatment with Biostimulators and Biologics

    Biostimulators work by stimulating the body’s natural processes, particularly collagen production.

    Biologics work by harnessing the regenerative potential of natural substances derived from human cells, tissues, or proteins to promote tissue repair, collagen synthesis, and overall skin renewal.

    Choosing the Right Approach

    Choosing between a biostimulator and a biologic for facial rejuvenation depends on various factors, including the patient’s individual goals, skin condition, preferences, and medical history.

    While both biostimulators and biologics offer significant benefits, they cater to different needs and preferences. Here are some considerations to help you make an informed decision:


    Desired Results: Biostimulators are effective for addressing concerns such as wrinkles, facial folds, and volume loss by stimulating collagen production and improving skin texture and firmness gradually over time.

    Longevity: Biostimulators offer long-lasting results, with effects typically lasting for months to years depending on the specific product used and individual patient response.

    Treatment Area: Biostimulators are suitable for larger treatment areas or areas requiring significant volume restoration, such as the cheeks, temples and jawline.

    Patient Preference for Synthetic Materials: If the patient prefers treatments that involve synthetic or biocompatible materials rather than natural substances derived from the body, biostimulators may be a preferred option.


    Enhanced Healing: Biologics can accelerate wound healing and tissue repair, making them ideal for patients with sensitive skin or those undergoing more invasive procedures.

    Natural Approach: Biologics offer a natural and holistic approach to facial rejuvenation by harnessing the body’s own regenerative potential.

    Immediate Results: Some biologics, such as PRP or growth factor-based treatments, may provide immediate improvements in skin texture, tone, and hydration, making them suitable for patients seeking quick results.

    Treatment Area and Concerns: Biologics are versatile and can address a wide range of concerns, including fine lines, wrinkles, scars, uneven skin tone and texture. They are suitable for various treatment areas, including the face, neck, décolletage and hands.

    Patient Preference for Natural Materials: If the patient prefers treatments that involve natural substances derived from the body and have minimal risk of adverse reactions, biologics may be the preferred option.


    Biologics and biostimulators are administered for facial rejuvenation through various techniques, including injections (both single needle and multi-needle automated injectors), topical applications and minimally invasive procedures.

    Close-up of the 9 pin needles on the MeDinjecT automated injector.
    Utilizing an automated injector for biostimulator and biologic injections in facial rejuvenation not only ensures precise and consistent delivery of the treatment but also minimizes discomfort, reduces the risk of bruising and optimizes the overall efficacy, providing patients with smoother, more natural-looking results.

    The specific method of administration depends on the type of biologic or biostimulator being used, as well as the patient’s treatment goals and preferences.

    Here’s an overview of common administration methods.


    Subcutaneous Injections: Biostimulators such as poly-L-lactic acid (PLLA), calcium hydroxylapatite (CaHA), and polycaprolactone (PCL) are typically administered through subcutaneous injections directly into the targeted areas of the face. The injections are usually performed using a fine needle or a multi-needle automated injector to minimize discomfort and ensure precise placement.

    Intradermal Injections: Biologics such as platelet-rich plasma (PRP) and growth factor-based treatments are commonly administered through intradermal injections. PRP injections involve drawing blood from the patient, processing it to isolate the platelet-rich plasma and then injecting it into the skin using a syringe with a fine needle or a multi-needle automated injector. Similarly, growth factor-based treatments may involve injecting growth factor solutions directly into the skin to stimulate collagen production and promote tissue regeneration.

    Minimally Invasive Procedures

    Microneedling with PRP: Microneedling, a minimally invasive procedure that involves creating micro-injuries in the skin using tiny needles, can be combined with PRP to enhance collagen induction and tissue repair. PRP is applied to the skin before or after microneedling to facilitate penetration and maximize the rejuvenating effects.

    Combination Injections: Biologics and biostimulators can be combined in the same treatment session to synergistically enhance results. For example, PRP injections may be combined with hyaluronic acid fillers or biostimulators to provide both immediate volume enhancement and long-term collagen stimulation.

    Administration with a Multi-Needle Automated Injector

    Using a multi-needle automated injector to administer biostimulators or biologics for facial rejuvenation offers several benefits that contribute to improved treatment outcomes and patient satisfaction.

    Here are some of the advantages:

    Precise and Consistent Delivery

    Uniform Distribution: Multi-needle automated injectors ensure that biologics are delivered evenly across the treatment area, minimizing the risk of uneven distribution and optimizing the effectiveness of the treatment.

    Consistent Depth: The automated injector controls the depth of penetration, ensuring that biologics are delivered to the desired depth within the skin for maximum efficacy. This consistency helps avoid variations in treatment results and reduces the risk of complications.

    Minimized Discomfort and Downtime

    Reduced Pain: Multi-needle automated injectors typically use fine needles and advanced technology to minimize discomfort during the injection process. Patients experience less pain compared to manual injections, enhancing their comfort and overall treatment experience.

    Quicker Procedure: The automated nature of multi-needle injectors allows for faster treatment times compared to manual injections. This not only improves patient convenience but also reduces the risk of discomfort and fatigue during longer procedures.

    Enhanced Safety and Precision

    Reduced Risk of Injury: Multi-needle automated injectors are equipped with safety features that minimize the risk of accidental needle sticks and injuries to both patients and healthcare providers. This enhances overall treatment safety and reduces the likelihood of adverse events.

    Precise Targeting: The ability to adjust parameters such as needle depth and injection speed allows for precise targeting of specific areas of concern, such as wrinkles, fine lines, or scars. This precision ensures that biologics are delivered exactly where they are needed for optimal results.

    Improved Treatment Outcomes

    Enhanced Efficacy: The controlled delivery of biologics by multi-needle automated injectors ensures that the active ingredients reach the target tissues at the appropriate depth and concentration, maximizing their therapeutic effects.

    Customizable Treatments: Multi-needle automated injectors offer flexibility in treatment parameters, allowing aesthetic providers to customize treatments according to each patient’s unique needs and goals. This tailored approach maximizes the likelihood of achieving desired outcomes and patient satisfaction.

    Providing Biostimulators and Biologics to Your Patients

    When it comes to facial rejuvenation, selecting the right treatment approach depends on various factors, including patient goals, skin condition, and medical history. Whether opting for biostimulators or biologics, personalizing the treatment to the patient is key to achieving optimal results.

    While biostimulators offer gradual improvement and long-lasting results, biologics may require multiple sessions to maintain the desired effect. Understanding the expected timeline and longevity of each treatment option can help aesthetic providers to make an informed decision based on their patient’s lifestyle and preferences.

    The digital display on the MeDinjecT automated injector.
    When it comes to administering biostimulators and biologics for facial rejuvenation, the MeDinjecT automated injector stands out as the optimal choice, offering unparalleled precision, comfort, and efficacy, ensuring patients receive the most effective treatment with minimal discomfort and downtime.

    In the ever-evolving landscape of facial rejuvenation, biostimulators and biologics represent two distinct yet complementary approaches to achieving youthful, radiant skin. Whether stimulating collagen production or tapping into the body’s regenerative potential, these innovative treatments offer safe, effective solutions for restoring confidence and revitalizing the complexion.

    Private Practice Solutions will happily answer any questions you have about biostimulators or biologics. 


    1. de Almeida AT, Figueredo V, da Cunha ALG, Casabona G, Costa de Faria JR, Alves EV, Sato M, Branco A, Guarnieri C, Palermo E. Consensus Recommendations for the Use of Hyperdiluted Calcium Hydroxyapatite (Radiesse) as a Face and Body Biostimulatory Agent. Plast Reconstr Surg Glob Open. 2019 Mar 14;7(3):e2160. doi: 10.1097/GOX.0000000000002160. PMID: 31044123; PMCID: PMC6467620.
    2. de Melo F, Nicolau P, Piovano L, Lin SL, Baptista-Fernandes T, King MI, Camporese A, Hong K, Khattar MM, Christen MO. Recommendations for volume augmentation and rejuvenation of the face and hands with the new generation polycaprolactone-based collagen stimulator (Ellansé®). Clin Cosmet Investig Dermatol. 2017 Nov 8;10:431-440. doi: 10.2147/CCID.S145195. PMID: 29184426; PMCID: PMC5685142.
    3. Attenello NH, Maas CS. Injectable fillers: review of material and properties. Facial Plast Surg. 2015 Feb;31(1):29-34. doi: 10.1055/s-0035-1544924. Epub 2015 Mar 12. PMID: 25763894.
    4. Avelar L, Ong A, Ong D, Wai ACS, Wai AYT, Sungkyu J, Seok LH, Tam E, Leng SE, Huang J, Chao Y, Bråsäter D, Prygova I. Consensus recommendations on the use of injectable poly-l-lactic acid in Asian patients. J Cosmet Dermatol. 2023 Dec;22(12):3223-3231. doi: 10.1111/jocd.15969. Epub 2023 Oct 2. PMID: 37786340.
    5. Shamban AT. Current and new treatments of photodamaged skin. Facial Plast Surg. 2009 Dec;25(5):337-46. doi: 10.1055/s-0029-1243083. Epub 2009 Dec 18. PMID: 20024876.
    6. Cameli N, Mariano M, Cordone I, Abril E, Masi S, Foddai ML. Autologous Pure Platelet-Rich Plasma Dermal Injections for Facial Skin Rejuvenation: Clinical, Instrumental, and Flow Cytometry Assessment. Dermatol Surg. 2017 Jun;43(6):826-835. doi: 10.1097/DSS.0000000000001083. PMID: 28375975.
    7. Massidda E. Starting Point for Protocols on the Use of Hyperdiluted Calcium Hydroxylapatite (Radiesse®) for Optimizing Age-Related Biostimulation and Rejuvenation of Face, Neck, Décolletage and Hands: A Case Series Report. Clin Cosmet Investig Dermatol. 2023 Nov 29;16:3427-3439. doi: 10.2147/CCID.S420068. PMID: 38050476; PMCID: PMC10693750.
    8. Viscomi B, Faria G, Hernandez CA, Perez L, Spada J, De la Fuente V, Muniz M. Contouring Plus: A Comprehensive Approach of the Lower Third of the Face with Calcium Hydroxylapatite and Hyaluronic Acid. Clin Cosmet Investig Dermatol. 2023 Apr 5;16:911-924. doi: 10.2147/CCID.S400605. PMID: 37041818; PMCID: PMC10083035.
    9. Ulusal BG. Platelet-rich plasma and hyaluronic acid – an efficient biostimulation method for face rejuvenation. J Cosmet Dermatol. 2017 Mar;16(1):112-119. doi: 10.1111/jocd.12271. Epub 2016 Sep 5. PMID: 27595866.
    10. Peng GL. Platelet-Rich Plasma for Skin Rejuvenation: Facts, Fiction, and Pearls for Practice. Facial Plast Surg Clin North Am. 2019 Aug;27(3):405-411. doi: 10.1016/j.fsc.2019.04.006. PMID: 31280855.
    11. Zarei F, Abbaszadeh A. Application of Cell Therapy for Anti-Aging Facial Skin. Curr Stem Cell Res Ther. 2019;14(3):244-248. doi: 10.2174/1574888X13666181113113415. PMID: 30421684.
    12. Felix Bravo B, Bezerra de Menezes Penedo L, de Melo Carvalho R, Amante Miot H, Calomeni Elias M. Improvement of Facial Skin Laxity by a Combined Technique With Hyaluronic Acid and Calcium Hydroxylapatite Fillers: A Clinical and Ultrasonography Analysis. J Drugs Dermatol. 2022 Jan 1;21(1):102-106. doi: 10.36849/JDD.2022.6333. PMID: 35005868.
    13. Ichihashi M, Tanaka M, Iizuka T, Totsuka H, Tominaga E, Hitomi Y, Ando H, Nishikata T, Mizutani KI. A Single Intradermal Injection of Autologous Adipose-Tissue-Derived Stem Cells Rejuvenates Aged Skin and Sharpens Double Eyelids. J Pers Med. 2023 Jul 20;13(7):1162. doi: 10.3390/jpm13071162. PMID: 37511775; PMCID: PMC10381540.
    14. Loghem JV, Yutskovskaya YA, Philip Werschler W. Calcium hydroxylapatite: over a decade of clinical experience. J Clin Aesthet Dermatol. 2015 Jan;8(1):38-49. PMID: 25610523; PMCID: PMC4295857.